Phos-Tio Phosphopeptides

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Protein phosphorylation is a highly important mechanism in cell signalling pathways for regulating protein function. In particular phosphorylation is the reversible addition of a phosphate group to a protein or other organic molecule resulting in a conformational change in the structure and causing activation or deactivation of protein’s function. Analysis of protein phosporylation is problematic as the concentration of any given phosphoprotein and the ionization efficiency of phosphopeptides for MS are relatively low. GL Sciences’ Titanium Dioxide (TiO2 or Titania) products offer a new efficiecy level in the field of purification and enrichment of phosphopeptides replacing IMAC as the primary sample preparation method prior to LC/MS analysis.

What makes GL Sciences’ Titanium Dioxide products unique?

GL Sciences’ produces Titanium Dioxide as highly spherical beads with the optimum ratio of crystal forms of TiO2. This unique formulation of titanium dioxide beads lead to a superior performace of GL Sciences’ Phos-TiO and MonoTip products.

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Technical Data

Principal of Phos-TiO Sample Enrichment Products:

Under acidic conditions phosphate groups are preferentially adsorbed to the surface of TiO beads are eluted under basic conditions.

Titansphere TiO Bulk Material

Titansphere TiO bulk sorbent media is available in particle sizes 5µm und 10µm in quantities of 500 mg. The Titanium Dioxide crystals are spherical with a pore size of 100Å. They are used in a pH range of 2-12.

Description			Part No.
Titansphere TiO 5µm - 500 mg			5020-75000
Titansphere TiO 10µm - 500 mg			5020-75010

Titansphere Phos-TiO Kit

The Titansphere Phos-Tio Kit contains tip-columns with titansphere media in the tip. The convenient 200µl tip provides 3 mg Tip material also offered as bulk and the 10 µl size tip 1 mg TiO. The kits also include waste and collection tubes as well as all required buffers.

Description			Part No.
Titansphere Phos-Tio Kit 1mg/10µL - 24 pcs			5010-21309
Titansphere Phos-Tio Kit 1mg/10µL - 96 pcs			5010-21310
Titansphere Phos-Tio Kit 3mg/200µL - 24 pcs			5010-21311
Titansphere Phos-Tio Kit 3mg/200µL - 96 pcs			5010-21312
Titansphere Phos-Tio Tip 10µL - 24 pcs			5010-21302
Titansphere Phos-Tio Tip 10µL - 96 pcs			5010-21303
Titansphere Phos-Tio Tip 200µL - 24 pcs			5010-21307
Titansphere Phos-Tio Tip 200µL - 96 pcs			5010-21308
Titansphere Phos-Tio Column 50mg/3mL - 25 pcs			5010-21290
Titansphere Phos-Tio Column 100mg/3mL - 25 pcs			5010-21291
Centrifuge Adaptor (10 µL; 200 µL tips) - 24 pcs			5010-21514

GL-Tip SDB und GL-Tip GC

Styrene divinylbenzene SDB and graphite carbon GC centrifuge-operated micropipette GL-Tips allow a higher retention of a wider range of phosphopeptides. GL Sciences GL-Tip SDB is more hydrophobic than C18 and GL-Tip GC retains more hydrophilic phosphopeptides. They are the ideal TiO-2 desalting products prior to LC/MS.

Description			Part No.
GL-Tip SDB 200µL - 96/Pck			7820-11200
GL-Tip GC 200µL - 96/Pck			7820-11201
GL-Tip Adaptor (resusable) - 1 Stück			5010-21514

MonoTip TiO

GL Sciences’ MonoTip Tio pipette-based columns contain silica monolith coated with nanoparticles of titanium dioxide. They are used for sample volumes of 50-200 µL with approximately 5µg of phosphopeptide bound per tip.

Description			Part No.
MonoTip TiO 200µL - 24 pcs			5010-21007
MonoTip TiO 200µL - 96 pcs			5010-21005

"Basierend aauf der Monolith Technologie, Merck KGaA, Darmstadt, Deutschland"

Phos-Tio Phosphopeptide